Bud Collyer and Joan Alexander also were the voices of Superman and Lois Lane on the Superman radio series respectively.
This was the first appearance in animation of Batman and his supporting cast.
In "The Joke's on Robin", Chief O'Hara announces the jack-in-the-box's display opening by saying "Jack will pop out" just before the Joker emerges. Jack Napier was a pseudonym sometimes given to him in the comics.
The Dollman's archer dolls wear green and are blonde, a possible reference to another of D.C. Comics' heroes, Green Arrow.